ss-ED-mCherry-GPI
(Plasmid #163680)

• Purpose: Exprsses the GPI-anchored luminal domain of ST6GAL1 with a mCherry tag in mammalian cells
• Depositing Lab: Lei Lu
• Publication: Sun et al J Cell Sci. 2021 Oct 15;134(20). pii: 272560. doi: 10.1242/jcs.258564. Epub 2021 Oct 21.

Backbone

• Vector backbone: pmoxGFP-N1
• Backbone manufacturer: E. Snapp (Addgene plasmid # 68070)
• Backbone size w/o insert (bp): 3958
• Total vector size (bp): 5965
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: The luminal domain of ST6 beta-galactoside alpha-2,6-sialyltransferase 1 with N-terminally tagged signal sequences
• Alt name: ST6GAL1
• Alt name: ST
• Species: H. sapiens (human)
• Insert Size (bp): 2007
• Mutation: This chimera contains the ecto-domain of ST. Its N-terminus was fused with a signal peptide and C-terminus was tagged with mCherry and GPI anchor.
• GenBank ID: BC040009
• Entrez Gene: ST6GAL1 (a.k.a. CDw75, SIAT1, ST6GalI, ST6N)
• Tags / Fusion Proteins:
  • mCherry (C terminal on insert)
  • GPI anchor (C terminal on insert)
  • signal sequences (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: XhoI (not destroyed)
• 3′ cloning site: NotI (not destroyed)
• 5′ sequencing primer: CMV-F
• 3′ sequencing primer: SV40-PArev

Resource Information

• Supplemental Documents:
  • ss-ED-mCherry-GPI.gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry