pAc5 dOrai
(Plasmid
#16368)
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 16368 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepAc5.1
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Backbone manufacturerInvitrogen
- Backbone size w/o insert (bp) 5371
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Vector typeInsect Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameOrai
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SpeciesD. melanogaster (fly)
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Entrez Geneolf186-F (a.k.a. BcDNA:LD03241, CG11430, CRACM1, Dmel\CG11430, dorai, dOrai, dOrai1, l(2)k11505, Olf186-F, Orai, SPH206)
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Tag
/ Fusion Protein
- V5 (C terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ sequencing primer AC5 (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pAc5 dOrai was a gift from Anjana Rao (Addgene plasmid # 16368 ; http://n2t.net/addgene:16368 ; RRID:Addgene_16368) -
For your References section:
Biochemical and functional characterization of Orai proteins. Gwack Y, Srikanth S, Feske S, Cruz-Guilloty F, Oh-hora M, Neems DS, Hogan PG, Rao A. J Biol Chem. 2007 Jun 1. 282(22):16232-43. 10.1074/jbc.M609630200 PubMed 17293345