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Addgene

TMD20
(Plasmid #163675)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 163675 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pmoxGFP-N1
  • Backbone manufacturer
    E. Snapp (Addgene plasmid # 68070)
  • Backbone size w/o insert (bp) 4695
  • Total vector size (bp) 5043
  • Modifications to backbone
    The original NdeI in pmoxGFP-N1 is abolished.
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    partial length ST6 beta-galactoside alpha-2,6-sialyltransferase 1 mutant
  • Alt name
    ST6GAL1
  • Alt name
    ST
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    348
  • Mutation
    For this partial length ST chimera, two restriction sites, SpeI and NdeI, were introduced into the TMD by silent muation and its TMD length is elongated to 20 amino acids by inserting Ala, Val and Leu residues.
  • GenBank ID
    BC040009
  • Entrez Gene
    ST6GAL1 (a.k.a. CDw75, SIAT1, ST6GalI, ST6N)
  • Tag / Fusion Protein
    • GFP (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site XhoI (not destroyed)
  • 3′ cloning site AgeI (not destroyed)
  • 5′ sequencing primer CMV-F
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    TMD20 was a gift from Lei Lu (Addgene plasmid # 163675 ; http://n2t.net/addgene:163675 ; RRID:Addgene_163675)

  • For your References section:

    A quantitative study of the Golgi retention of glycosyltransferases. Sun X, Mahajan D, Chen B, Song Z, Lu L. J Cell Sci. 2021 Oct 15;134(20). pii: 272560. doi: 10.1242/jcs.258564. Epub 2021 Oct 21. 10.1242/jcs.258564 PubMed 34533190
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