SSF-GFP
(Plasmid #163656)

• Purpose: Expresses a TFR and ST6GAL1 swapped chimera with the cytosolic tail and TMD from ST6GAL1, and luminal domain from TFR in mammalian cells
• Depositing Lab: Lei Lu
• Publication: Sun et al J Cell Sci. 2021 Oct 15;134(20). pii: 272560. doi: 10.1242/jcs.258564. Epub 2021 Oct 21.

Backbone

• Vector backbone: pmoxGFP-N1
• Backbone manufacturer: E. Snapp (Addgene plasmid # 68070)
• Backbone size w/o insert (bp): 4700
• Total vector size (bp): 6803
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: ST6 beta-galactoside alpha-2,6-sialyltransferase 1 and transferrin receptor
• Alt name: ST6GAL1 and TFR
• Species: H. sapiens (human)
• Insert Size (bp): 2103
• Mutation: This chimera contains the cytosolic tail from ST, TMD from ST and luminal domain from TFR.
• GenBank ID: BC040009 NM_001128148
• Entrez Gene: ST6GAL1 (a.k.a. CDw75, SIAT1, ST6GalI, ST6N)
• Entrez Gene: TFRC (a.k.a. CD71, IMD46, T9, TFR, TFR1, TR, TRFR, p90)
• Tag / Fusion Protein:
  • GFP (C terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: XhoI (not destroyed)
• 3′ cloning site: BamHI (not destroyed)
• 5′ sequencing primer: CMV-F

Resource Information

• Supplemental Documents:
  • SSF-GFP.gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry