Man2a1-GFP
(Plasmid #163649)

• Purpose: Expresses GFP tagged Man2a1 in mammalian cells
• Depositing Lab: Lei Lu
• Publication: Sun et al J Cell Sci. 2021 Oct 15;134(20). pii: 272560. doi: 10.1242/jcs.258564. Epub 2021 Oct 21.

Backbone

• Vector backbone: pDmyc-neo-N1
• Backbone manufacturer: Dr. Lu Lei's lab
• Backbone size w/o insert (bp): 6171
• Total vector size (bp): 9600
• Modifications to backbone: The myc tag in this vector was replaced with GFP tag using SacII/NotI digestion.
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: mannosidase alpha class 2A member 1
• Alt name: Man2a1
• Species: H. sapiens (human)
• Insert Size (bp): 3429
• Mutation: Silent mutation was made to introduce a XbaI restriction site in Man2a1
• GenBank ID: BC142696
• Entrez Gene: MAN2A1 (a.k.a. AMan II, GOLIM7, MANA2, MANII)
• Tag / Fusion Protein:
  • GFP (C terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: XhoI (not destroyed)
• 3′ cloning site: SacII (not destroyed)
• 5′ sequencing primer: CMV-F or T7

Resource Information

• Supplemental Documents:
  • Man2a1-GFP.gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry