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Addgene

Man1B1-Myc
(Plasmid #163645)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 163645 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pDmyc-neo-N1
  • Backbone manufacturer
    Dr. Lu Lei's lab
  • Backbone size w/o insert (bp) 5541
  • Total vector size (bp) 7638
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    mannosidase alpha class 1B member 1
  • Alt name
    Man1B1
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    2097
  • GenBank ID
    BC063300
  • Entrez Gene
    MAN1B1 (a.k.a. ERMAN1, ERManI, MANA-ER, MRT15)
  • Tag / Fusion Protein
    • myc (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site XhoI (not destroyed)
  • 3′ cloning site XbaI (not destroyed)
  • 5′ sequencing primer CMV-F or T7
  • 3′ sequencing primer T3
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Man1B1-Myc was a gift from Lei Lu (Addgene plasmid # 163645 ; http://n2t.net/addgene:163645 ; RRID:Addgene_163645)

  • For your References section:

    A quantitative study of the Golgi retention of glycosyltransferases. Sun X, Mahajan D, Chen B, Song Z, Lu L. J Cell Sci. 2021 Oct 15;134(20). pii: 272560. doi: 10.1242/jcs.258564. Epub 2021 Oct 21. 10.1242/jcs.258564 PubMed 34533190
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