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Addgene

A3G-BE4.4
(Plasmid #163636)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 163636 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCMV
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    A3G-BE4.4
  • Species
    Synthetic; ; Cas9 is from S. pyogenes; A3G is from the H. sapiens
  • Insert Size (bp)
    5426
  • Promoter CMV
  • Tags / Fusion Proteins
    • BPSV40 NLS (N terminal on backbone)
    • BPSV40 NLS (C terminal on backbone)

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    A3G-BE4.4 was a gift from Xue Gao (Addgene plasmid # 163636 ; http://n2t.net/addgene:163636 ; RRID:Addgene_163636)

  • For your References section:

    Single C-to-T substitution using engineered APOBEC3G-nCas9 base editors with minimum genome- and transcriptome-wide off-target effects. Lee S, Ding N, Sun Y, Yuan T, Li J, Yuan Q, Liu L, Yang J, Wang Q, Kolomeisky AB, Hilton IB, Zuo E, Gao X. Sci Adv. 2020 Jul 15;6(29):eaba1773. doi: 10.1126/sciadv.aba1773. eCollection 2020 Jul. 10.1126/sciadv.aba1773 PubMed 32832622
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