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Addgene

plRL19
(Plasmid #163634)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 163634 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    N/A
  • Backbone manufacturer
    Rock Lab
  • Backbone size w/o insert (bp) 3088
  • Total vector size (bp) 4204
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    Shake at 220rpm. For best yield, grow for >16 hours.
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    L5 Integrase
  • Alt name
    L5 Int
  • Species
    Mycobacteriophage L5
  • Insert Size (bp)
    1116
  • GenBank ID
    M65194.1
  • Promoter Mycobacterial Optimized Promoter (MOP)

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    plRL19 was a gift from Jeremy Rock (Addgene plasmid # 163634 ; http://n2t.net/addgene:163634 ; RRID:Addgene_163634)

  • For your References section:

    Genome-wide gene expression tuning reveals diverse vulnerabilities of M. tuberculosis. Bosch B, DeJesus MA, Poulton NC, Zhang W, Engelhart CA, Zaveri A, Lavalette S, Ruecker N, Trujillo C, Wallach JB, Li S, Ehrt S, Chait BT, Schnappinger D, Rock JM. Cell. 2021 Jul 16. pii: S0092-8674(21)00824-2. doi: 10.1016/j.cell.2021.06.033. 10.1016/j.cell.2021.06.033 PubMed 34297925
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