plenti_DCLK1 G511N
(Plasmid
#163626)
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PurposeExpresses DCLK1 G511N in E. coli
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 163626 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepLenti6
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Vector typeLentiviral
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth TemperatureRoom Temperature
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Growth Strain(s)NEB Stable
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameDCLK1
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SpeciesH. sapiens (human)
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MutationG511N
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Entrez GeneDCLK1 (a.k.a. CL1, CLICK1, DCAMKL1, DCDC3A, DCLK)
- Promoter sv40
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Tag
/ Fusion Protein
- V5 (C terminal on insert)
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer ATGTCCTTCGGCAGAGACATG-3’ (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please note that the Addgene NGS sequence differs from the depositor's annotated sequence. These differences do not affect plasmid function.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
plenti_DCLK1 G511N was a gift from Kenneth Westover (Addgene plasmid # 163626 ; http://n2t.net/addgene:163626 ; RRID:Addgene_163626) -
For your References section:
Chemical Biology Toolkit for DCLK1 Reveals Connection to RNA Processing. Liu Y, Ferguson FM, Li L, Kuljanin M, Mills CE, Subramanian K, Harshbarger W, Gondi S, Wang J, Sorger PK, Mancias JD, Gray NS, Westover KD. Cell Chem Biol. 2020 Oct 15;27(10):1229-1240.e4. doi: 10.1016/j.chembiol.2020.07.011. Epub 2020 Aug 4. 10.1016/j.chembiol.2020.07.011 PubMed 32755567