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Addgene

plenti_DCLK1
(Plasmid #163625)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 163625 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pLenti6
  • Vector type
    Lentiviral

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    Room Temperature
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    DCLK1
  • gRNA/shRNA sequence
    unknown
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    507
  • Entrez Gene
    DCLK1 (a.k.a. CL1, CLICK1, DCAMKL1, DCDC3A, DCLK)
  • Promoter sv40
  • Tag / Fusion Protein
    • V5 (C terminal on insert)

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please note that the Addgene NGS sequence differs from the depositor's annotated sequence. These differences do not affect plasmid function.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    plenti_DCLK1 was a gift from Kenneth Westover (Addgene plasmid # 163625 ; http://n2t.net/addgene:163625 ; RRID:Addgene_163625)

  • For your References section:

    Chemical Biology Toolkit for DCLK1 Reveals Connection to RNA Processing. Liu Y, Ferguson FM, Li L, Kuljanin M, Mills CE, Subramanian K, Harshbarger W, Gondi S, Wang J, Sorger PK, Mancias JD, Gray NS, Westover KD. Cell Chem Biol. 2020 Oct 15;27(10):1229-1240.e4. doi: 10.1016/j.chembiol.2020.07.011. Epub 2020 Aug 4. 10.1016/j.chembiol.2020.07.011 PubMed 32755567
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