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pEGFP-N1-mSNX27 RNAi-resistant
(Plasmid #163620)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 163620 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pEGFP-N1
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    mouse SNX27 RNAi-resistant
  • Species
    M. musculus (mouse)
  • Mutation
    RNAi resistance
  • Entrez Gene
    Snx27 (a.k.a. 5730552M22Rik, D13Mgi2, ESTM4, ESTM45, ESTM47, R75405)
  • Tag / Fusion Protein
    • GFP (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Unknown (unknown if destroyed)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

fw primer RNAi resistant rat: GGGCAGCTGGAGAACCAAGTGATCGCATTCGAATGGGATGAGATGC

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pEGFP-N1-mSNX27 RNAi-resistant was a gift from Josef Kittler (Addgene plasmid # 163620 ; http://n2t.net/addgene:163620 ; RRID:Addgene_163620)

  • For your References section:

    SNX27-Mediated Recycling of Neuroligin-2 Regulates Inhibitory Signaling. Halff EF, Szulc BR, Lesept F, Kittler JT. Cell Rep. 2019 Nov 26;29(9):2599-2607.e6. doi: 10.1016/j.celrep.2019.10.096. 10.1016/j.celrep.2019.10.096 PubMed 31775031
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