pEGFP-N1-mSNX27-H112A
(Plasmid
#163619)
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Purposemouse SNX27 cDNA with a point mutation H112A C-terminally tagged with GFP
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 163619 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepEGFP-N1
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namemouse SNX27-H112A
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SpeciesM. musculus (mouse)
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MutationH112A
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Entrez GeneSnx27 (a.k.a. 5730552M22Rik, D13Mgi2, ESTM4, ESTM45, ESTM47, R75405)
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Tag
/ Fusion Protein
- GFP (C terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site Unknown (unknown if destroyed)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pEGFP-N1-mSNX27-H112A was a gift from Josef Kittler (Addgene plasmid # 163619 ; http://n2t.net/addgene:163619 ; RRID:Addgene_163619) -
For your References section:
SNX27-Mediated Recycling of Neuroligin-2 Regulates Inhibitory Signaling. Halff EF, Szulc BR, Lesept F, Kittler JT. Cell Rep. 2019 Nov 26;29(9):2599-2607.e6. doi: 10.1016/j.celrep.2019.10.096. 10.1016/j.celrep.2019.10.096 PubMed 31775031