pET28a-His6-OAZ-GSY-SPM-Ctag
(Plasmid
#163613)
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PurposeExpresses truncated human Ornithine Decarboxylase Antizyme fused to SPM in bacterial cells for calcium-induced covalent coupling to Ornithine Decarboxylase.
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 163613 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepET-28a
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Backbone manufacturerNovagen
- Backbone size w/o insert (bp) 5246
- Total vector size (bp) 6416
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameOAZ-GSY-SPM
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SpeciesSynthetic
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Insert Size (bp)1170
- Promoter T7
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Tags
/ Fusion Proteins
- His6 (N terminal on backbone)
- C-tag (C terminal on insert)
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer T7F
- 3′ sequencing primer T7R (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pET28a-His6-OAZ-GSY-SPM-Ctag was a gift from Mark Howarth (Addgene plasmid # 163613 ; http://n2t.net/addgene:163613 ; RRID:Addgene_163613) -
For your References section:
NeissLock provides an inducible protein anhydride for covalent targeting of endogenous proteins. Scheu AHA, Lim SYT, Metzner FJ, Mohammed S, Howarth M. Nat Commun. 2021 Jan 29;12(1):717. doi: 10.1038/s41467-021-20963-5. 10.1038/s41467-021-20963-5 PubMed 33514717
