pET28a-SENP8 (full length))
(Plasmid #16361)

• Depositing Lab: Guy Salvesen
• Publication: Mikolajczyk et al J Biol Chem. 2007 Sep 7. 282(36):26217-24.

Backbone

• Vector backbone: pET28a
• Backbone manufacturer: Novagen
• Backbone size w/o insert (bp): 5369
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Growth instructions: BL21(DE3)Codon plus
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: human SENP8 full length
• Alt name: SENP8 - SUMO/sentrin specific peptidase 8
• Species: H. sapiens (human)
• Insert Size (bp): 663
• Mutation: human SENP8 full length, see Depositor Comments
• Entrez Gene: SENP8 (a.k.a. DEN1, NEDP1, PRSC2)
• Tag / Fusion Protein:
  • His (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NdeI (not destroyed)
• 3′ cloning site: XhoI (not destroyed)
• 5′ sequencing primer: T7
• 3′ sequencing primer: T7

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Sanford-Burnham Medical Research Institute Ancillary Agreement
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Please note: Plasmid contains a K211T mutation in SENP8 compared to the NCBI reference sequence AAH31411.1. It is not known how this mutation affects plasmid function.