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Addgene

pET28a-SENP5 (catalytic domain)
(Plasmid #16358)

Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 16358 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pET28a
  • Backbone manufacturer
    Novagen
  • Backbone size w/o insert (bp) 5369
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    BL21(DE3)Codon plus
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    human SENP5 catalytic domain
  • Alt name
    SENP5 - SUMO/sentrin specific peptidase 5
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    600
  • Mutation
    Amino Acid 567 to 755 catalytic domain of human SENP5.
  • Entrez Gene
    SENP5
  • Tag / Fusion Protein
    • His (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NdeI (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer T7
  • (Common Sequencing Primers)

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET28a-SENP5 (catalytic domain) was a gift from Guy Salvesen (Addgene plasmid # 16358 ; http://n2t.net/addgene:16358 ; RRID:Addgene_16358)
  • For your References section:

    Small ubiquitin-related modifier (SUMO)-specific proteases: profiling the specificities and activities of human SENPs. Mikolajczyk J, Drag M, Bekes M, Cao JT, Ronai Z, Salvesen GS. J Biol Chem. 2007 Sep 7. 282(36):26217-24. 10.1074/jbc.M702444200 PubMed 17591783