pCMV_UdgX-EE-UdgX-NG-nCas9-UdgX
(Plasmid
#163572)
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PurposeMammalian CG-to-GC base editing
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 163572 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepCMV
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Vector typeMammalian Expression, CRISPR
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameUdgX-EE-UdgX-NG-nCas9-UdgX
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SpeciesH. sapiens (human)
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MutationnCas9 (D10A)EE (R126E, R132E)NG (L111R, D1135V, G1218R, E1219F, A1322R, R1335V, T1337R)
Cloning Information
- Cloning method Gibson Cloning
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCMV_UdgX-EE-UdgX-NG-nCas9-UdgX was a gift from David Liu (Addgene plasmid # 163572 ; http://n2t.net/addgene:163572 ; RRID:Addgene_163572) -
For your References section:
Efficient C*G-to-G*C base editors developed using CRISPRi screens, target-library analysis, and machine learning. Koblan LW, Arbab M, Shen MW, Hussmann JA, Anzalone AV, Doman JL, Newby GA, Yang D, Mok B, Replogle JM, Xu A, Sisley TA, Weissman JS, Adamson B, Liu DR. Nat Biotechnol. 2021 Jun 28. pii: 10.1038/s41587-021-00938-z. doi: 10.1038/s41587-021-00938-z. 10.1038/s41587-021-00938-z PubMed 34183861