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Addgene

pCMV_EE-nCas9
(Plasmid #163541)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 163541 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCMV
  • Vector type
    Mammalian Expression, CRISPR

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    EE-nCas9
  • Species
    H. sapiens (human)
  • Mutation
    nCas9 (D10A)EE (R126E, R132E)

Cloning Information

  • Cloning method Gibson Cloning

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCMV_EE-nCas9 was a gift from David Liu (Addgene plasmid # 163541 ; http://n2t.net/addgene:163541 ; RRID:Addgene_163541)

  • For your References section:

    Efficient C*G-to-G*C base editors developed using CRISPRi screens, target-library analysis, and machine learning. Koblan LW, Arbab M, Shen MW, Hussmann JA, Anzalone AV, Doman JL, Newby GA, Yang D, Mok B, Replogle JM, Xu A, Sisley TA, Weissman JS, Adamson B, Liu DR. Nat Biotechnol. 2021 Jun 28. pii: 10.1038/s41587-021-00938-z. doi: 10.1038/s41587-021-00938-z. 10.1038/s41587-021-00938-z PubMed 34183861
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