eGFP-ova-C1
(Plasmid #163524)

• Purpose: Expresses both eGFP and chicken ovalbumin in mammalian cells
• Depositing Lab: Thomas Weber
• Publication: Expression plasmid for production of murine IFN gamma (unpublished)

Backbone

• Vector backbone: eGFP-C1
• Backbone manufacturer: Clonetech
• Backbone size w/o insert (bp): 4731
• Total vector size (bp): 5973
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: enhanced-GFP-chicken ovalbumin tandem
• Species: G. gallus (chicken)
• Insert Size (bp): 1161
• Promoter: CMV
• Tag / Fusion Protein:
  • eGFP (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: Kpn1 (unknown if destroyed)
• 3′ cloning site: BamH1 (unknown if destroyed)
• 5′ sequencing primer: CTTCTTCAAGTCCGCCATGCCCGAAGGC
• 3′ sequencing primer: TTATCTAGATCCGGTGGATCC

Resource Information

• Supplemental Documents:
  • eGFP-c1-clonetech.ape

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Cloned by ananda Mookerjee and Thomas Weber