pGEM-Oct4
(Plasmid #16352)

• Depositing Lab: James Thomson
• Publication: Yu et al Science. 2007 Dec 21. 318(5858):1917-20.

Backbone

• Vector backbone: pGEM-T Easy
• Backbone manufacturer: Promega
• Backbone size w/o insert (bp): 3000
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Homo sapiens POU domain, class 5, transcription factor 1 (POU5F1)
• Alt name: POU5F1
• Alt name: Oct4
• Species: H. sapiens (human)
• Insert Size (bp): 1100
• GenBank ID: NM_002701
• Entrez Gene: POU5F1 (a.k.a. OCT3, OCT4, OTF-3, OTF3, OTF4, Oct-3, Oct-4, Oct3/4)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ sequencing primer: M13 Reverse
• 3′ sequencing primer: T7

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

This plasmid contains the Oct4 sequence that was cloned into the lentivirus plasmid and used in cell reprogramming.