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Addgene

mu-IFNg-pCIneo
(Plasmid #163517)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 163517 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCIneo
  • Backbone manufacturer
    Promega
  • Modifications to backbone
    Changed multiple cloning site
  • Vector type
    Mammalian Expression, Bacterial Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    murine-IFN gamma
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    548
  • GenBank ID
    NM_008337
  • Entrez Gene
    Ifng (a.k.a. IFN-g, If2f, Ifg)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Xho1 (unknown if destroyed)
  • 3′ cloning site Not1 (unknown if destroyed)
  • 5′ sequencing primer CGTTTAGTGAACCGTCAGATC
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Plasmid created by Ananda Mookerjee and Thomas Weber

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    mu-IFNg-pCIneo was a gift from Thomas Weber (Addgene plasmid # 163517 ; http://n2t.net/addgene:163517 ; RRID:Addgene_163517)
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