CN1525-rAAV-hsA2-eHGT_079h-minRho-SYFP2-WPRE3-BGHpA
(Plasmid
#163501)
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PurposeDirect-expressing SYFP2 AAV Virus
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Depositing Labs
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 163501 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonerAAV-hsA2-eHGT_079h-minRho-SYFP2-WPRE3-BGHpA
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Backbone manufacturerAllen Institute
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Vector typeAAV
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameSYFP2
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SpeciesSynthetic
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Insert Size (bp)720
- Promoter MinRho
Cloning Information
- Cloning method Ligation Independent Cloning
- 5′ sequencing primer AGCGCACGAGGGAGCTT
- 3′ sequencing primer ACGTCGCCGTCCAGCTC (Common Sequencing Primers)
Resource Information
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Funded by BRAIN initiative.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
CN1525-rAAV-hsA2-eHGT_079h-minRho-SYFP2-WPRE3-BGHpA was a gift from The Allen Institute for Brain Science & Boaz Levi (Addgene plasmid # 163501 ; http://n2t.net/addgene:163501 ; RRID:Addgene_163501) -
For your References section:
Functional enhancer elements drive subclass-selective expression from mouse to primate neocortex. Mich JK, Graybuck LT, Hess EE, Mahoney JT, Kojima Y, Ding Y, Somasundaram S, Miller JA, Kalmbach BE, Radaelli C, Gore BB, Weed N, Omstead V, Bishaw Y, Shapovalova NV, Martinez RA, Fong O, Yao S, Mortrud M, Chong P, Loftus L, Bertagnolli D, Goldy J, Casper T, Dee N, Opitz-Araya X, Cetin A, Smith KA, Gwinn RP, Cobbs C, Ko AL, Ojemann JG, Keene CD, Silbergeld DL, Sunkin SM, Gradinaru V, Horwitz GD, Zeng H, Tasic B, Lein ES, Ting JT, Levi BP. Cell Rep. 2021 Mar 30;34(13):108754. doi: 10.1016/j.celrep.2021.108754. 10.1016/j.celrep.2021.108754 PubMed 33789096
