pGEM-LIN28
(Plasmid #16350)

• Depositing Lab: James Thomson
• Publication: Yu et al Science. 2007 Dec 21. 318(5858):1917-20.

Backbone

• Vector backbone: pGEM-T Easy
• Backbone manufacturer: Promega
• Backbone size w/o insert (bp): 3000
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Homo sapiens lin-28 homolog (C. elegans)
• Alt name: LIN28
• Species: H. sapiens (human)
• Insert Size (bp): 600
• GenBank ID: NM_024674
• Entrez Gene: LIN28A (a.k.a. CSDD1, LIN-28, LIN28, ZCCHC1, lin-28A)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ sequencing primer: T7
• 3′ sequencing primer: M13 Reverse

Resource Information

• Articles Citing this Plasmid:
  • 3 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

This plasmid contains the Lin28 sequence that was cloned into the lentivirus plasmid and used in cell reprogramming.