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CN1621-rAAV-hsA2-eHGT_128h-minRho-SYFP2-WPRE3-BGHpA
(Plasmid #163498)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 163498 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    rAAV-hsA2-eHGT_128h-minRho-SYFP2-WPRE3-BGHpA
  • Backbone manufacturer
    Allen Institute
  • Vector type
    AAV

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    SYFP2
  • Species
    Synthetic
  • Insert Size (bp)
    720
  • Promoter MinRho

Cloning Information

  • Cloning method Ligation Independent Cloning
  • 5′ sequencing primer AGCGCACGAGGGAGCTT
  • 3′ sequencing primer ACGTCGCCGTCCAGCTC
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Funded by BRAIN initiative.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    CN1621-rAAV-hsA2-eHGT_128h-minRho-SYFP2-WPRE3-BGHpA was a gift from The Allen Institute for Brain Science & Boaz Levi (Addgene plasmid # 163498 ; http://n2t.net/addgene:163498 ; RRID:Addgene_163498)

  • For your References section:

    Functional enhancer elements drive subclass-selective expression from mouse to primate neocortex. Mich JK, Graybuck LT, Hess EE, Mahoney JT, Kojima Y, Ding Y, Somasundaram S, Miller JA, Kalmbach BE, Radaelli C, Gore BB, Weed N, Omstead V, Bishaw Y, Shapovalova NV, Martinez RA, Fong O, Yao S, Mortrud M, Chong P, Loftus L, Bertagnolli D, Goldy J, Casper T, Dee N, Opitz-Araya X, Cetin A, Smith KA, Gwinn RP, Cobbs C, Ko AL, Ojemann JG, Keene CD, Silbergeld DL, Sunkin SM, Gradinaru V, Horwitz GD, Zeng H, Tasic B, Lein ES, Ting JT, Levi BP. Cell Rep. 2021 Mar 30;34(13):108754. doi: 10.1016/j.celrep.2021.108754. 10.1016/j.celrep.2021.108754 PubMed 33789096
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