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Addgene

AiP1051-pAAV-Synapsin promoter-oNigri-WPRE-hGHpA
(Plasmid #163489)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 163489 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pAAV-Synapsin promoter-Cre-WPRE-bGHpA
  • Backbone manufacturer
    Allen Institute
  • Vector type
    AAV

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    oNigri
  • Species
    Synthetic
  • Insert Size (bp)
    1401
  • Promoter Synapsin promoter

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site KpnI (not destroyed)
  • 5′ sequencing primer CACCACCTGTCAGCTCCTTT
  • 3′ sequencing primer AAGGGAGATCCGACTCGTCT
  • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Funded by BRAIN initiative.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    AiP1051-pAAV-Synapsin promoter-oNigri-WPRE-hGHpA was a gift from The Allen Institute for Brain Science & Tanya Daigle (Addgene plasmid # 163489 ; http://n2t.net/addgene:163489 ; RRID:Addgene_163489)