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RCP83
(Plasmid #163466)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 163466 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    RCP83
  • Backbone manufacturer
    Gingko Bioworks
  • Backbone size w/o insert (bp) 4540
  • Modifications to backbone
    none
  • Vector type
    Bacterial Expression, Yeast Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert 1

  • Gene/Insert name
    Promoter fragment (Sharon2012_1922)

Gene/Insert 2

  • Gene/Insert name
    His3 minimal promoter
  • Species
    S. cerevisiae (budding yeast)

Gene/Insert 3

  • Gene/Insert name
    yeGFP

Resource Information

  • Supplemental Documents
  • A portion of this plasmid was derived from a plasmid made by
    Gingko Bioworks

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Promoter fragment (Sharon2012_1922) is designed from Sharon et al., Nat Biotechnol. 2012 May 20;30(6):521-30. DOI: 10.1038/nbt.2205

Digestion with SfiI will remove the inserted promoter and yeGFP

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    RCP83 was a gift from Frank Albert (Addgene plasmid # 163466 ; http://n2t.net/addgene:163466 ; RRID:Addgene_163466)

  • For your References section:

    Systematic identification of cis-regulatory variants that cause gene expression differences in a yeast cross. Renganaath K, Cheung R, Day L, Kosuri S, Kruglyak L, Albert FW. Elife. 2020 Nov 12;9. pii: 62669. doi: 10.7554/eLife.62669. 10.7554/eLife.62669 PubMed 33179598
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