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Addgene

HIV7-Bcl2-CD28-CD3z-T2A-EGFR
(Plasmid #163465)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 163465 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    HIV7
  • Backbone size w/o insert (bp) 6853
  • Vector type
    Lentiviral

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Bim-specific CAR
  • Alt name
    Bcl2-long_spacer-CD28tm-CD28icd-CD3zicd-T2A-EGFRt
  • Species
    H. sapiens (human), Synthetic
  • Insert Size (bp)
    3006
  • Tag / Fusion Protein
    • HA (N terminal on insert)

Cloning Information

  • Cloning method Gibson Cloning

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    HIV7-Bcl2-CD28-CD3z-T2A-EGFR was a gift from David Baker (Addgene plasmid # 163465 ; http://n2t.net/addgene:163465 ; RRID:Addgene_163465)

  • For your References section:

    Designed protein logic to target cells with precise combinations of surface antigens. Lajoie MJ, Boyken SE, Salter AI, Bruffey J, Rajan A, Langan RA, Olshefsky A, Muhunthan V, Bick MJ, Gewe M, Quijano-Rubio A, Johnson J, Lenz G, Nguyen A, Pun S, Correnti CE, Riddell SR, Baker D. Science. 2020 Sep 25;369(6511):1637-1643. doi: 10.1126/science.aba6527. Epub 2020 Aug 20. 10.1126/science.aba6527 PubMed 32820060
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