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pGEM mouse PEA3 [TJ#200]
(Plasmid #16346)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 16346 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pGEM
  • Backbone manufacturer
    Promega
  • Backbone size w/o insert (bp) 3000
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    PEA3
  • Alt name
    Pea3
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    2300
  • Entrez Gene
    Etv4 (a.k.a. AW414408, Pea-3, Pea3)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site XbaI (not destroyed)
  • 3′ cloning site BamHI (not destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer SP6
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Mouse full-length PEA3 cDNA in pGEM. To make an antisense probe, cut with XbaI or ApaI and transcribe with SP6 (ApaI cuts away the cloning artifact - see map). To make a sense probe, cut with BamHI and transcribe with T7.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pGEM mouse PEA3 [TJ#200] was a gift from Thomas Jessell (Addgene plasmid # 16346 ; http://n2t.net/addgene:16346 ; RRID:Addgene_16346)
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