pLJC6-GPT2-3xFLAG
(Plasmid #163449)

• Purpose: lentiviral overexpression vector for human GPT2
• Depositing Lab: Jason Cantor
• Publication: Rossiter et al Cell Metab. 2021 Feb 23. pii: S1550-4131(21)00061-9. doi: 10.1016/j.cmet.2021.02.005.

Backbone

• Vector backbone: pLJC6
• Vector type: Mammalian Expression, Lentiviral
• Selectable markers: Blasticidin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: GPT2
• Species: H. sapiens (human)
• Mutation: See: comments
• Entrez Gene: GPT2 (a.k.a. ALT2, GPT 2, MRT49, NEDSPM)
• Promoter: Ubc
• Tag / Fusion Protein:
  • 3xFLAG (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: PacI (not destroyed)
• 3′ cloning site: NotI (not destroyed)
• 5′ sequencing primer: GTAAATTGTCCGCTAAATTCTGGC
• 3′ sequencing primer: CCCTTTTCTTTTAAAATTGTGGATGAATACTGCC

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Insert sequence contains 20 silent mutations introduced to: (I) remove an internal NotI site; (II) reduce the GC content at the 5' terminus of the gene; and (III) abolish recognition by sgGPT2_5