pET28_KPNA1
(Plasmid
#163440)
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PurposeBacterial expression of His-tagged KPNA1 in the pET28 protein expression backbone
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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 163440 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepET28 a (+)
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameKPNA1
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Alt nameRCH2
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Alt nameSRP1
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SpeciesH. sapiens (human)
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Entrez GeneKPNA1 (a.k.a. IPOA5, NPI-1, RCH2, SRP1)
- Promoter T7
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Tags
/ Fusion Proteins
- 6xHis (N terminal on insert)
- 6xHis (C terminal on insert)
Cloning Information
- Cloning method Gibson Cloning
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pET28_KPNA1 was a gift from Sanford Simon (Addgene plasmid # 163440)