BFP-RanQ69L
(Plasmid #163438)

• Purpose: Mammalian expression of RanQ69L with tag-BFP; contains miniAux domain for degredation
• Depositing Lab: Sanford Simon
• Publication: Simon lab plasmids (unpublished)

Backbone

• Vector backbone: pmCherry-C1
• Backbone manufacturer: Clontech
• Backbone size w/o insert (bp): 4700
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: RanQ69L
• Alt name: RAs-related Nuclear protein Q69L
• Alt name: Ran
• Species: H. sapiens (human)
• Mutation: Q69L
• Entrez Gene: RAN (a.k.a. ARA24, Gsp1, TC4)
• Promoter: CMV
• Tag / Fusion Protein:
  • tag-BFP (C terminal on insert)

Cloning Information

• Cloning method: Gibson Cloning

Resource Information

• Supplemental Documents:
  • BFP-RanQ69L.gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Evrogen Limited Use Label License for FPs
• Industry Terms:
  • Not Available to Industry