Nup54-mEGFP494(flex1)
(Plasmid
#163430)
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PurposeMammalian expression of Nup54 with a mEGFP (missing the first 5 amino acids) at amino acid position 494 with a flexible linker of 6 amino acids at the carboxyl end of the mEGFP
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Depositing Lab
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Publication
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 163430 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backboneUnspecified
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameNup54
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SpeciesH. sapiens (human)
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Entrez GeneNUP54
- Promoter CMV
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Tag
/ Fusion Protein
- mEGFP (C terminal on insert)
Cloning Information
- Cloning method Gibson Cloning
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
Nup54-mEGFP494(flex1) was a gift from Sanford Simon (Addgene plasmid # 163430 ; http://n2t.net/addgene:163430 ; RRID:Addgene_163430)
