Nup58_mEGFP(-8)
(Plasmid
#163425)
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PurposeMammalian expression of Nup58 with a mEGFP (missing the first 8 amino acids) at amino acid position 412
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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 163425 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backboneUnspecified
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameNup58
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Alt nameNUP45
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Alt nameNUPL1
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SpeciesH. sapiens (human)
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Entrez GeneNUPL1 (a.k.a. PRO2463, KIAA0410)
- Promoter CMV
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Tag
/ Fusion Protein
- mEGFP (C terminal on insert)
Cloning Information
- Cloning method Gibson Cloning
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
Nup58_mEGFP(-8) was a gift from Sanford Simon (Addgene plasmid # 163425)