pcDNA5-TO-PV-NLS-Dsred
(Plasmid #16341)

• Depositing Lab: Anton Bennett
• Publication: Rodrigues et al J Biol Chem. 2007 Jun 8. 282(23):17061-8.

Backbone

• Vector backbone: pcDNA5/TO
• Backbone manufacturer: Invitrogen
• Backbone size w/o insert (bp): 5700
• Vector type: Mammalian Expression
• Selectable markers: Hygromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: PV-DsRed-NLS
• Alt name: Parvalbumin
• Species: R. norvegicus (rat)
• Insert Size (bp): 1100
• GenBank ID: M12725
• Entrez Gene: Pvalb (a.k.a. PALB1, Pva)
• Tags / Fusion Proteins:
  • NLS (C terminal on insert)
  • Myc (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: KpnI (not destroyed)
• 3′ cloning site: XbaI (not destroyed)
• 5′ sequencing primer: CMV Forword

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry