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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 16340 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepcDNA5/TO
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Backbone manufacturerInvitrogen
- Backbone size w/o insert (bp) 5700
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Vector typeMammalian Expression
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Selectable markersHygromycin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameDsRed
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Insert Size (bp)700
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Mutation5'Prime Site: Not I 3'Primer Site : Not I Stul Release 1.3Kb fragment
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site Not I (not destroyed)
- 3′ cloning site Not I (not destroyed)
- 5′ sequencing primer CMV forward priming site
- 3′ sequencing primer BGH Reverse priming site (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
5'Primer Site: Not I;
3'Primer Site: Not I;
Stul Release 1.3Kb fragment
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pcDNA5-TO-DsRed was a gift from Anton Bennett (Addgene plasmid # 16340 ; http://n2t.net/addgene:16340 ; RRID:Addgene_16340) -
For your References section:
Nucleoplasmic calcium is required for cell proliferation. Rodrigues MA, Gomes DA, Leite MF, Grant W, Zhang L, Lam W, Cheng YC, Bennett AM, Nathanson MH. J Biol Chem. 2007 Jun 8. 282(23):17061-8. 10.1074/jbc.M700490200 PubMed 17420246