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HA-Clover-HIF-2alpha T528E
(Plasmid #163375)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 163375 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    HA-Clover-HIF-2_ Wildtype
  • Backbone manufacturer
    Leonard Daly
  • Backbone size w/o insert (bp) 6163
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Hypoxia Inducible Factor 2 alpha
  • Alt name
    HIF-2a
  • Alt name
    ESAP1
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    2613
  • Mutation
    T528E
  • Entrez Gene
    EPAS1 (a.k.a. ECYT4, HIF2A, HLF, MOP2, PASD2, bHLHe73)
  • Promoter CMV Promoter
  • Tag / Fusion Protein
    • HA- and Clover-tagged (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BsrGI (not destroyed)
  • 3′ cloning site NotI (not destroyed)
  • 5′ sequencing primer GCAGAACACCCCCATCGG
  • 3′ sequencing primer CAGTACCAGGACTACAGC
    • (Common Sequencing Primers)

Resource Information

  • Supplemental Documents
  • A portion of this plasmid was derived from a plasmid made by
    HIF2A gene from HaloTag-HIF-2a plasmid (obtained from the Katzusa DNA Research Institute)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Site-directed Mutagenesis

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    HA-Clover-HIF-2alpha T528E was a gift from Violaine Sée (Addgene plasmid # 163375 ; http://n2t.net/addgene:163375 ; RRID:Addgene_163375)
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