Nanog5P reporter
(Plasmid #16337)

• Depositing Lab: Austin Cooney
• Publication: Gu et al Mol Cell Biol. 2005 Oct . 25(19):8507-19.

Backbone

• Vector backbone: pGL3-basic
• Backbone manufacturer: Promega
• Backbone size w/o insert (bp): 4800
• Vector type: Mammalian Expression, Luciferase

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Nanog promoter
• Species: M. musculus (mouse)
• Insert Size (bp): 2500
• Entrez Gene: Nanog (a.k.a. 2410002E02Rik, ENK, Stm1, ecat4)
• Promoter: Mouse Nanog promoter
• Tag / Fusion Protein:
  • Luciferase (C terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: KpnI (not destroyed)
• 3′ cloning site: XhoI (not destroyed)
• 5′ sequencing primer: RVprimer3
• 3′ sequencing primer: LucNrev

Resource Information

• Articles Citing this Plasmid:
  • 12 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Luciferase Limited Use Label License
• Industry Terms:
  • Not Available to Industry

Depositor Comments

5' Nanog Luciferase reporter, contains 2.5 kb of 5' promoter region of the mouse Nanog gene.