HA-Clover-HIF-1alpha Wildtype
(Plasmid #163365)

• Purpose: Express HA- and Clover-tagged HIF-1alpha wildtype plasmid
• Depositing Lab: Violaine Sée
• Publication: See lab plasmids (unpublished)

Backbone

• Vector backbone: HA-HIF1alpha-pcDNA3
• Backbone manufacturer: William Kaelin (Addgene plasmid # 18949)
• Backbone size w/o insert (bp): 7937
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Hypoxia Inducible Factor 1 alpha
• Species: H. sapiens (human)
• Insert Size (bp): 2481
• Mutation: Wildtype
• Entrez Gene: HIF1A (a.k.a. HIF-1-alpha, HIF-1A, HIF-1alpha, HIF1, HIF1-ALPHA, MOP1, PASD8, bHLHe78)
• Promoter: CMV Promoter
• Tag / Fusion Protein:
  • HA- and Clover-tagged (N terminal on backbone)

Cloning Information

• Cloning method: Ligation Independent Cloning
• 5′ sequencing primer: AACGGGACTTTCCAAAATG
• 3′ sequencing primer: GAACAGAATGGAATGGAGC

Resource Information

• Supplemental Documents:
  • HA-Clover-HIF1a (wt).gb
• A portion of this plasmid was derived from a plasmid made by: Clover-GFP gene from pcDNA3-Clover from Michael Lin (Addgene plasmid # 40259)

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry