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NLuc CMV-Neo Flexi Vector pFC32K SARS-CoV2 5'Nsp1
(Plasmid #163319)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 163319 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pFC32K
  • Backbone manufacturer
    Promega
  • Total vector size (bp) 5694
  • Vector type
    Mammalian Expression, Luciferase
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Nsp1
  • Species
    Severe acute respiratory syndrome coronavirus 2
  • Insert Size (bp)
    540
  • GenBank ID
    43740578 YP_009724389.1
  • Entrez Gene
    ORF1ab (a.k.a. GU280_gp01)
  • Tag / Fusion Protein
    • NanoLuc (C terminal on backbone)

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    NLuc CMV-Neo Flexi Vector pFC32K SARS-CoV2 5'Nsp1 was a gift from Dawn Chandler (Addgene plasmid # 163319 ; http://n2t.net/addgene:163319 ; RRID:Addgene_163319)
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