NLuc CMV-Neo Flexi Vector pFC32K SARS-CoV2 5'Nsp1 PRF
(Plasmid
#163317)
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PurposeEncodes for first COVID protein - NSP1, NSP10, NSP11, and fragment of NSP12 fused to nano-luciferase
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 163317 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepFC32K
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Backbone manufacturerPromega
- Total vector size (bp) 5694
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Vector typeMammalian Expression, Luciferase
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameNsp1, Nsp10, Nsp11 ,Nsp12
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SpeciesSevere acute respiratory syndrome coronavirus 2
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Insert Size (bp)960
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GenBank ID43740578 YP_009724389.1
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Entrez GeneORF1ab (a.k.a. GU280_gp01)
- Promoter CMV
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Tag
/ Fusion Protein
- NanoLuc (C terminal on backbone)
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer T7
- 3′ sequencing primer SV40 promoter (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
NLuc CMV-Neo Flexi Vector pFC32K SARS-CoV2 5'Nsp1 PRF was a gift from Dawn Chandler (Addgene plasmid # 163317 ; http://n2t.net/addgene:163317 ; RRID:Addgene_163317)
Map uploaded by the depositor.
