xfz2N/pCS2+
(Plasmid #16329)

• Depositing Lab: Peter Klein
• Publication: Deardorff et al Mech Dev. 1999 Sep . 87(1-2):229-33.

Backbone

• Vector backbone: pCS2+
• Backbone size w/o insert (bp): 4095
• Vector type: Vertebrate expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: frizzled-2
• Alt name: fz2N
• Alt name: Nxfz2
• Species: X. laevis (frog)
• Insert Size (bp): 600
• Mutation: Extracellular ligand binding domain of Xenopus frizzled-2.
• Entrez Gene: fzd2 (a.k.a. frizzled2, xfz2)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI (not destroyed)
• 5′ sequencing primer: SP6
• 3′ sequencing primer: T3

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Secreted Wnt inhibitor.
For sense transcripts, cut with NotI and transcribe with SP6.

Please note that the cloning strategy converted phenylalanine-229 to a tyrosine (F229Y), just before the termination codon. According to the depositing scientist, this change is unlikely to affect function.