xfz3N/pCS2+
(Plasmid #16328)

• Depositing Lab: Peter Klein
• Publication: Deardorff et al Development. 2001 Oct . 128(19):3655-63.

Backbone

• Vector backbone: pCS2+
• Backbone size w/o insert (bp): 4095
• Vector type: Vertebrate expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: frizzled-3
• Alt name: fz3
• Alt name: Nxfz3
• Alt name: N3
• Species: X. laevis (frog)
• Insert Size (bp): 700
• Mutation: Extracellular ligand binding domain of Xenopus frizzled-3.
• Entrez Gene: fzd3.L (a.k.a. XELAEV_18028255mg, frizzled3, frz-3, frz3, fz3, fzd3)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ sequencing primer: SP6

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

This construct contains the amino terminus of xfz3, which includes the signal sequence, cysteine-rich domain (CRD) and the linker region. It terminates just before the first transmembrane region.
For sense transcripts, cut with NotI and transcribe with SP6.