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Addgene

F6CI10
(Plasmid #163276)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 163276 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET21a(+)
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    F6CI10
  • Alt name
    flavoprotein oxidoreductase
  • Alt name
    (S)-2-hydroxy-acid oxidase
  • Species
    Desulfofundulus kuznetsovii
  • Promoter T7
  • Tags / Fusion Proteins
    • T7 (N terminal on backbone)
    • 10xHis (C terminal on backbone)

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Insert name is a UniProt identifier

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    F6CI10 was a gift from Martin Engqvist (Addgene plasmid # 163276 ; http://n2t.net/addgene:163276 ; RRID:Addgene_163276)

  • For your References section:

    Experimental and computational investigation of enzyme functional annotations uncovers misannotation in the EC 1.1.3.15 enzyme class. Rembeza E, Engqvist MKM. PLoS Comput Biol. 2021 Sep 23;17(9):e1009446. doi: 10.1371/journal.pcbi.1009446. eCollection 2021 Sep. 10.1371/journal.pcbi.1009446 PubMed 34555022
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