xfz3/pCS2+
(Plasmid
#16327)
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 16327 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepCS2+
- Backbone size w/o insert (bp) 4095
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Vector typeVertebrate expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namefrizzled-3
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Alt namefz3
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Alt namexfz3
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Alt nameXfz3/pCS2(III)
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SpeciesX. laevis (frog)
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Insert Size (bp)1900
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GenBank IDAJ001754
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Entrez Genefzd3.L (a.k.a. XELAEV_18028255mg, frizzled3, frz-3, frz3, fz3, fzd3)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site EcoRI (not destroyed)
- 3′ cloning site SpeI (not destroyed)
- 5′ sequencing primer SP6
- 3′ sequencing primer T3 (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Removed 5'UTR from xfz3.
For sense transcripts, cut with NotI and transcribe with SP6.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
xfz3/pCS2+ was a gift from Peter Klein (Addgene plasmid # 16327 ; http://n2t.net/addgene:16327 ; RRID:Addgene_16327) -
For your References section:
A role for frizzled 3 in neural crest development. Deardorff MA, Tan C, Saint-Jeannet JP, Klein PS. Development. 2001 Oct . 128(19):3655-63. PubMed 11585792
Map uploaded by the depositor.
