xfz7N/pCS2+
(Plasmid #16325)

• Depositing Lab: Peter Klein
• Publication: Deardorff et al Development. 1998 Jul . 125(14):2687-700.

Backbone

• Vector backbone: pCS2+
• Backbone size w/o insert (bp): 4095
• Vector type: Vertebrate expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: frizzled-7
• Alt name: fz7
• Alt name: xfz7
• Species: X. laevis (frog)
• Insert Size (bp): 1700
• Mutation: Amino terminal end
• Entrez Gene: fzd7.L (a.k.a. XELAEV_18044942mg, Fz-7, Xfz7, frizzled-7, frizzled7, frz-7, frz7, fz7, fzd7, fzd7-a, fzd7-b)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI (not destroyed)
• 5′ sequencing primer: SP6

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Contains xfz7 cysteine rich extracellular domain, up to 1st TM. This construct was made from Nxfz7myc. The myc tag was removed. The insert has a BamHI site at the 5' end. The myc tag was removed as a ClaI-XbaI fragment and the ends were blunted, so there are no 3' sites to cut it out. For sense transcripts, cut with NotI.