xfz8 probe/pBS
(Plasmid
#16323)
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Depositing Lab
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Publication
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Sequence Information
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Please contact us if you would like Addgene to sequence a portion of this plasmid before you request it.
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Full plasmid sequence is not available for this item.
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 16323 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepBluescript II KS
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Backbone manufacturerStratagene
- Backbone size w/o insert (bp) 3000
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namefrizzled-8
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Alt namefz8
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Alt namexfz8
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SpeciesX. laevis (frog)
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Insert Size (bp)2100
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MutationBase pairs 1159-3240 of xfz8 cDNA
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Entrez Genefzd8.S (a.k.a. XELAEV_18032854mg, FZD8, Fz-8, frizzled8, fz8, fzd8-a, fzd8-b, xfz8)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ sequencing primer T3
- 3′ sequencing primer T7 (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Contains bases 1159-3240 of xfz8 cDNA, from just after TM2 to polyA.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
xfz8 probe/pBS was a gift from Peter Klein (Addgene plasmid # 16323 ; http://n2t.net/addgene:16323 ; RRID:Addgene_16323) -
For your References section:
Frizzled-8 is expressed in the Spemann organizer and plays a role in early morphogenesis. Deardorff MA, Tan C, Conrad LJ, Klein PS. Development. 1998 Jul . 125(14):2687-700. PubMed 9636083