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Addgene

xfz8 probe/pBS
(Plasmid #16323)

Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 16323 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pBluescript II KS
  • Backbone manufacturer
    Stratagene
  • Backbone size w/o insert (bp) 3000
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    frizzled-8
  • Alt name
    fz8
  • Alt name
    xfz8
  • Species
    X. laevis (frog)
  • Insert Size (bp)
    2100
  • Mutation
    Base pairs 1159-3240 of xfz8 cDNA
  • Entrez Gene
    fzd8.S (a.k.a. XELAEV_18032854mg, FZD8, Fz-8, frizzled8, fz8, fzd8-a, fzd8-b, xfz8)

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Contains bases 1159-3240 of xfz8 cDNA, from just after TM2 to polyA.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    xfz8 probe/pBS was a gift from Peter Klein (Addgene plasmid # 16323 ; http://n2t.net/addgene:16323 ; RRID:Addgene_16323)
  • For your References section:

    Frizzled-8 is expressed in the Spemann organizer and plays a role in early morphogenesis. Deardorff MA, Tan C, Conrad LJ, Klein PS. Development. 1998 Jul . 125(14):2687-700. PubMed 9636083