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Addgene

xfz8N/pCS2+
(Plasmid #16322)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 16322 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCS2+
  • Backbone size w/o insert (bp) 4095
  • Vector type
    Vertebrate expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    frizzled-8
  • Alt name
    xfz8N
  • Alt name
    Nxfz8
  • Alt name
    N8
  • Species
    X. laevis (frog)
  • Insert Size (bp)
    730
  • Mutation
    Extracellular ligand binding domain of frizzled-8. Secreted wnt inhibitor.
  • Entrez Gene
    fzd8.S (a.k.a. XELAEV_18032854mg, FZD8, Fz-8, frizzled8, fz8, fzd8-a, fzd8-b, xfz8)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site XhoI (destroyed during cloning)
  • 5′ sequencing primer SP6
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

This construct contains the amino terminus of xfz8, which includes the signal sequence, cysteine-rich domain (CRD) and the linker region. It terminates just before the first transmembrane region. This plasmid does not contain a myc tag and is potent at causing enlarged cement gland and shortened axes. This construct was generated by removing a ClaI-XhoI fragment from Nxfz8/CS2MT. For sense transcripts, cut with NotI.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    xfz8N/pCS2+ was a gift from Peter Klein (Addgene plasmid # 16322 ; http://n2t.net/addgene:16322 ; RRID:Addgene_16322)

  • For your References section:

    Frizzled-8 is expressed in the Spemann organizer and plays a role in early morphogenesis. Deardorff MA, Tan C, Conrad LJ, Klein PS. Development. 1998 Jul . 125(14):2687-700. PubMed 9636083
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