Skip to main content
Addgene

xfz8/pCS2+
(Plasmid #16321)

Print

Full plasmid sequence is not available for this item.

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 16321 Standard format: Plasmid sent in bacteria as agar stab 1 $85
Add to Cart

Backbone

  • Vector backbone
    pCS2+
  • Backbone size w/o insert (bp) 4095
  • Vector type
    Vertebrate expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    frizzled-8
  • Alt name
    fz8
  • Alt name
    xfz8
  • Species
    X. laevis (frog)
  • Insert Size (bp)
    1800
  • Entrez Gene
    fzd8.S (a.k.a. XELAEV_18032854mg, FZD8, Fz-8, frizzled8, fz8, fzd8-a, fzd8-b, xfz8)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer SP6
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

For sense transcripts, cut with NotI.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    xfz8/pCS2+ was a gift from Peter Klein (Addgene plasmid # 16321 ; http://n2t.net/addgene:16321 ; RRID:Addgene_16321)

  • For your References section:

    Frizzled-8 is expressed in the Spemann organizer and plays a role in early morphogenesis. Deardorff MA, Tan C, Conrad LJ, Klein PS. Development. 1998 Jul . 125(14):2687-700. PubMed 9636083
Related items:
Commonly requested with: