tau/pET29b
(Plasmid #16316)

• Depositing Lab: Peter Klein
• Publication: Hedgepeth et al Dev Biol. 1997 May 1. 185(1):82-91.

Backbone

• Vector backbone: pET29b
• Backbone manufacturer: EMD Biosciences
• Backbone size w/o insert (bp): 5370
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: tau
• Alt name: tau protein
• Alt name: Tau T40
• Alt name: tau transcript variant 2
• Species: H. sapiens (human)
• Insert Size (bp): 1400
• GenBank ID: NM_005910
• Entrez Gene: MAPT (a.k.a. DDPAC, FTD1, FTDP-17, MAPTL, MSTD, MTBT1, MTBT2, PPND, PPP1R103, TAU, Tau-PHF6, tau-40)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NdeI (not destroyed)
• 3′ cloning site: EcoRI (not destroyed)
• 5′ sequencing primer: T7 promoter
• 3′ sequencing primer: T7 terminator

Resource Information

• Addgene Notes:
  • Protocol for Expression
• Articles Citing this Plasmid:
  • 19 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

For bacterial expression of human tau T40.