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pENTR E2 HSPter-RPS5Ap-mito
(Plasmid #163146)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 163146 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    PINII
  • Vector type
    Entry vector

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    A terminator of Arabidopsis heat shock protein, RPS5A promoter, and mitochondrial presequence
  • Species
    Synthetic

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Multisite Gateway entry vector to terminate the E1 ORF and having RPS5A promoter with mitochondrial presequence for the E3 ORF. Use with Platinum Gate TALEN kit (Kit # 1000000043) and plasmids #158728-#158735

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pENTR E2 HSPter-RPS5Ap-mito was a gift from Shin-ichi Arimura (Addgene plasmid # 163146 ; http://n2t.net/addgene:163146 ; RRID:Addgene_163146)

  • For your References section:

    Targeted gene disruption of ATP synthases 6-1 and 6-2 in the mitochondrial genome of Arabidopsis thaliana by mitoTALENs. Arimura SI, Ayabe H, Sugaya H, Okuno M, Tamura Y, Tsuruta Y, Watari Y, Yanase S, Yamauchi T, Itoh T, Toyoda A, Takanashi H, Tsutsumi N. Plant J. 2020 Oct 24. doi: 10.1111/tpj.15041. 10.1111/tpj.15041 PubMed 33098708
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