pLyGo-Bs-2
(Plasmid
#163140)
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Purpose(Empty Backbone) pLyGo cloning vector for a sequence of interest (LPMO) in Bacillus subtilis. Vector encoding the AmyQ signal peptide and the LyGo cassette (SapI-ccdB-SapI)
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 163140 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepBS293C-amyE
- Backbone size (bp) 6610
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Vector typeBacterial Expression, Synthetic Biology
- Promoter PamyL-PamyQ-PcryIIIA-cryIIIAstab49
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Selectable markersChloramphenicol
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)ccdB Survival
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Copy numberLow Copy
Cloning Information
- Cloning method Restriction Enzyme
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please visit https://www.biorxiv.org/content/10.1101/2020.11.04.368555v1 for bioRxiv preprint.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pLyGo-Bs-2 was a gift from Morten Norholm (Addgene plasmid # 163140 ; http://n2t.net/addgene:163140 ; RRID:Addgene_163140) -
For your References section:
LyGo: A Platform for Rapid Screening of Lytic Polysaccharide Monooxygenase Production. Hernandez-Rollan C, Falkenberg KB, Rennig M, Bertelsen AB, Ipsen JO, Brander S, Daley DO, Johansen KS, Norholm MHH. ACS Synth Biol. 2021 Apr 16;10(4):897-906. doi: 10.1021/acssynbio.1c00034. Epub 2021 Apr 2. 10.1021/acssynbio.1c00034 PubMed 33797234
Map uploaded by the depositor.
